concentration | |||||
---|---|---|---|---|---|
reagent | stock | final | volume | comment | |
reagent | stock | final | volume | comment | |
PEG 400 | 100% | 30% | 60 | uL | |
Quick Ligase buffer (NEB, USA) | 10x | 2x | 45 | uL | |
ATP | 10mM | 1.75mM | 35 | uL | |
EndIt enzyme (Epicentre USA) | 16 | uL | |||
Fast Ligase (Epicentre, USA) | 16 | uL | |||
EGTA | 0.5M | 40mM | 16 | uL | |
dNTPs | 10mM | 0.5mM | 10 | uL | |
sodium butyrate | 1M | 10mM | 2 | uL |
concentration | |||||
---|---|---|---|---|---|
reagent | stock | final | volume | comment | |
Triton® X-100 | 10% | 2% | 20 | mL | |
Tris-HCl | 1M, pH 7.5 | 100mM | 10 | mL | |
NaCl | 5M | 300mM | 6 | mL | |
sodium deoxycholate | 5% | 0.20% | 4 | mL | |
CaCl2 | 1M | 10mM | 1 | mL | |
H2O | 59 | mL | |||
sodium butyrate | 1M | 10mM | 25uL in 2.5mL of 2X buffer | ||
Micrococcal Nuclease | 100% | 0.04% | 1uL in 2.5mL of 2X buffer |
concentration | |||||
---|---|---|---|---|---|
reagent | stock | final | volume | comment | |
digestion buffer | 2x | 0.5x | 250 | mL | |
PEG 400 | 100% | 18% | 175 | mL | |
EGTA | 0.5M | 20mM | 40 | mL | |
PBS | 1x | 0.54x | 535 | mL |
concentration | |||||
---|---|---|---|---|---|
reagent | stock | final | volume | comment | |
Triton® X-100 | 10% | 1% | 10 | mL | |
EGTA | 0.5M | 30mM | 6 | mL | |
EDTA | 0.5M | 30mM | 6 | mL | |
Tris-HCl | 1M, pH 7.5 | 50mM | 5 | mL | |
NaCl | 5M | 150mM | 3 | mL | |
sodium deoxycholate | 5% | 0.10% | 2 | mL | |
H2O | 68 | mL |
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